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Image Search Results
Journal: bioRxiv
Article Title: A Sex-Specific Switch in Platelet Receptor Signaling Following Myocardial Infarction
doi: 10.1101/580415
Figure Lengend Snippet: Platelets from healthy women and men were isolated and stimulated ex vivo with agonists for (A) PARl (TRAP-6), (B) the thromboxane receptor (U46619), and (C) the P2Y 12 receptor (ADP). Platelet activation was assessed by FACS for alpha granule secretion (surface p-select in) as mean fold increase from baseline ± SEM. * P <0.05 between groups at the indicated time point by the Kruskal-Wallis test followed by Dunn’s post test correction.
Article Snippet:
Techniques: Isolation, Ex Vivo, Activation Assay
Journal: bioRxiv
Article Title: A Sex-Specific Switch in Platelet Receptor Signaling Following Myocardial Infarction
doi: 10.1101/580415
Figure Lengend Snippet: Blood was drawn from women and men as they were diagnosed with STEMI or NSTEMI, prior to coronary angiography, and prior to loading with a P2Y 12 receptor antagonist. Platelet rich plasma was isolated and platelets were stimulated for 15 mins with an agonist for: PARl (TRAP-6, 10 µM), the P2Y 12 receptor (ADP, 10 µM), or the thromboxane receptor {U46619, 10 µM). Platelets were labeled with a tagged antibody for P selectin, and then analyzed by flow cytometry. Platelet function is represented as median fold change in surface P-selectin as mean fluorescence intensity (MFI) from baseline± 95% C. I. Differences between women and men for each agonist was assessed by the M ann-Whitney U test. *P=0.0001. **P=0.0473. NS=Not significant.
Article Snippet:
Techniques: Isolation, Labeling, Flow Cytometry, Fluorescence
Journal: bioRxiv
Article Title: A Sex-Specific Switch in Platelet Receptor Signaling Following Myocardial Infarction
doi: 10.1101/580415
Figure Lengend Snippet: Blood was drawn from healthy women and men and compared to blood drawn from patients with Ml (STEMI + NSTEMI) as soon as they were diagnosed (prior to coronary angiography, and prior to loading with a P2Y 12 receptor antagonist). Platelet rich plasma was isolated and platelets were stimulated for 15 mins with an agonist for platelet PARl (TRAP-6, 10 µM) or the P2Y 12 receptor (ADP, 10 µM). Platelets were labeled with a tagged antibody for p-selectin, and then analyzed by flow cytometry. Platelet function is represented as mean fold change in surface P-selectin by median fluorescence intensity (MFI) from baseline± 95% C.I. Differences between women and men for each agonist was assessed by the M ann-Whitney U test. Level of significance is noted above the graph.
Article Snippet:
Techniques: Isolation, Labeling, Flow Cytometry, Fluorescence
Journal: bioRxiv
Article Title: A Sex-Specific Switch in Platelet Receptor Signaling Following Myocardial Infarction
doi: 10.1101/580415
Figure Lengend Snippet: Blood was drawn patients as soon as they were diagnosed with NSTEMI, prior to coronary angiography, and prior to loading with a P2Y 12 receptor antagonist. Platelet rich plasma was isolated and platelets were stimulated for 15 mins with an agonist for PARl (TRAP - 6, 10 µM). Platelets were labeled with a FITC-tagged antibody for PARl, and analyzed by flow cytometry. Platelet surface receptor density is represented as mean fluorescence intensity (MFI) ± SEM. Differences between groups was assessed by the Kruskal-Wallis test followed by Dunn ’s post test. Level of significance is noted above the graph.
Article Snippet:
Techniques: Isolation, Labeling, Flow Cytometry, Fluorescence